Wang Xue-yan, Ma Wen-jiang, Liu Chang-shan, Li Ying-xin
Department of Paediatric, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China.
Lasers Med Sci. 2014 May;29(3):1043-50. doi: 10.1007/s10103-013-1456-5. Epub 2013 Oct 26.
Asthma is a complex chronic inflammatory disease of the airways that involves the activation of many inflammatory and other types of cells. We investigated the effect of low-level laser therapy (LLLT) on allergic asthma in rats and compared its effect with that of the glucocorticoid budesonide. Asthma was induced by challenge and repeated exposure to ovalbumin. Asthmatic rats were then treated with LLLT or budesonide suspension. LLLT at 8 J/cm(2) once daily for 21 days could relieve pathological damage and airway inflammation in asthmatic rats. LLLT could decrease the total numbers of cells and eosinophils in bronchoalveolar lavage fluid. LLLT could reduce levels of IL-4 and increase IFN-γ levels in bronchoalveolar lavage fluid and serum, meanwhile reduce serum IgE levels. Flow cytometry assay showed that LLLT can regulate the Th1/Th2 imbalance of asthmatic rats. LLLT had a similar effect to that of budesonide. These findings suggest that the mechanism of LLLT treatment of asthma is by adjustment of Th1/Th2 imbalance. Thus, LLLT could take over some of the effects of budesonide for the treatment of asthma, thereby reducing some of the side effects of budesonide.
哮喘是一种复杂的气道慢性炎症性疾病，涉及多种炎症细胞和其他类型细胞的激活。我们研究了低强度激光疗法（LLLT）对大鼠过敏性哮喘的影响，并将其与糖皮质激素布地奈德的效果进行比较。通过激发和反复暴露于卵清蛋白诱导哮喘。然后用LLLT或布地奈德混悬液治疗哮喘大鼠。以8 J/cm²的剂量每天一次进行LLLT治疗，持续21天，可减轻哮喘大鼠的病理损伤和气道炎症。LLLT可减少支气管肺泡灌洗液中的细胞总数和嗜酸性粒细胞数量。LLLT可降低支气管肺泡灌洗液和血清中IL-4的水平，同时提高IFN-γ水平，降低血清IgE水平。流式细胞术检测显示，LLLT可调节哮喘大鼠的Th1/Th2失衡。LLLT的效果与布地奈德相似。这些发现表明，LLLT治疗哮喘的机制是通过调节Th1/Th2失衡。因此，LLLT可替代布地奈德的部分治疗哮喘的作用，从而减少布地奈德的一些副作用。
