Zhang Bo, Lu Lu, Zhang Xuejin, Ye Weiyuan, Wu Jun, Xi Qiliang, Zhang Xuejun
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
J Mol Neurosci. 2014 Jul;53(3):335-44. doi: 10.1007/s12031-013-0136-z. Epub 2013 Oct 26.
MiR-132 is enriched in the central nerve system and is thought to be involved in neuronal development, maturation and function, and to be associated with several neurological disorders including Alzheimer's disease. In addition to its documented neuronal functions, an emerging role for miR-132 in tumorigenesis has been suggested. Recently, hsa-miR-132 was shown to be modulated in different tumor types. However, its role in non-small cell lung cancer (NSCLC) remains unclear. Here, we show that hsa-miR-132 can initiate apoptosis in NSCLC cells to dramatically attenuate tumor formation in nude mice independent of its effect on the proliferation/apoptosis-associated gene, acetylcholinesterase (AChE). Interestingly, hsa-miR-132 has no pro-apoptotic effect in normal pulmonary trachea epithelium. Taken together, these results suggest that hsa-miR-132 represses NSCLC growth by inducing apoptosis independent of AChE.
微小RNA-132（MiR-132）在中枢神经系统中富集，被认为参与神经元的发育、成熟和功能，并与包括阿尔茨海默病在内的多种神经系统疾病有关。除了已记录的神经元功能外，有研究表明MiR-132在肿瘤发生中也发挥着新的作用。最近，有研究显示人源微小RNA-132（hsa-miR-132）在不同肿瘤类型中受到调控。然而，其在非小细胞肺癌（NSCLC）中的作用仍不清楚。在此，我们表明hsa-miR-132可引发NSCLC细胞凋亡，从而显著减弱裸鼠体内的肿瘤形成，且这一作用独立于其对增殖/凋亡相关基因乙酰胆碱酯酶（AChE）的影响。有趣的是，hsa-miR-132对正常肺气管上皮细胞没有促凋亡作用。综上所述，这些结果表明hsa-miR-132通过诱导凋亡来抑制NSCLC生长，且该作用独立于AChE。
