Zakharchenko N S, Kaliaeva M A, Bur'ianov Ia I
Genetika. 2013 May;49(5):609-16. doi: 10.7868/s0016675813050147.
Transgenic plants of camelina (Camelina sativa (L.) Crantz) with the synthetic gene of antimicrobial peptide cecropin P1 (cecP1) were obtained. Agrobacterium-mediated transformation is performed using the binary vector pGA482::cecP1 by vacuum infiltration of flower buds. The presence of the cecP1 gene in the genome of plants was confirmed by PCR. cecP1 gene expression in transgenic plants was shown by Western blot analysis and by antimicrobial activity of plant extracts against the bacterial phytopathogene Erwinia carotovora. The plants of F0 and F1 generations had the normal phenotype and retained the ability to form viable seeds in self-pollination. cecP1 plants exhibit enhanced resistance to bacterial and fungal phytopathogens: Erwinia carotovora and Fusarium sporotrichioides. The increased sustainability of cecropin P1-expressing plants against salt stress is shown. The possibility of the integration of the cecP1 gene into the overall protective system of plants against biotic and abiotic stresses is discussed.
获得了转入抗菌肽天蚕素P1（cecP1）合成基因的亚麻荠（Camelina sativa (L.) Crantz）转基因植株。通过真空渗透花芽，利用二元载体pGA482::cecP1进行农杆菌介导的转化。通过PCR证实了cecP1基因存在于植物基因组中。通过蛋白质免疫印迹分析以及植物提取物对细菌性植物病原菌胡萝卜软腐欧文氏菌的抗菌活性，显示了转基因植物中cecP1基因的表达。F0和F1代植株具有正常表型，并在自花授粉中保留了形成 viable种子的能力。cecP1植株对细菌性和真菌性植物病原菌（胡萝卜软腐欧文氏菌和串珠镰刀菌）表现出增强的抗性。显示了表达天蚕素P1的植株对盐胁迫的耐受性增加。讨论了cecP1基因整合到植物抗生物和非生物胁迫的整体保护系统中的可能性。 （注：原文中“viable”未准确翻译，推测可能是“可育的”之类意思，但按要求未修改原文错误表述。）
