Brassica and Oilseeds Research Department, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK.
Theor Appl Genet. 1996 Oct;93(5-6):1006-10. doi: 10.1007/BF00224105.
The biochemical and genetical relationship between aliphatic glucosinolates which have methylthioalkyl, methylsulphinylalkyl and alkenyl side chains has not been resolved by biochemical studies. In this study, two hypothetical models are tested by the genetic analysis of a backcross population between Brassica drepanensis and B. atlantica. The results support one of the models in which 3-methylthiopropyl glucosinolate is sequentially converted to 3-methylsulphinylpropyl, and then to 2-propenyl glucosinolate, by the action of dominant alleles at two loci. RFLP mapping positioned both loci on the same linkage group homologous to the B. napus N19 linkage group. The implication of the results for the genetic manipulation of glucosinolates in Brassica to improve flavour and nutritional properties, and in order to investigate plant-insect interactions, is discussed.
脂肪族硫代葡萄糖苷的生物化学和遗传学关系，其侧链具有甲基硫烷基、甲基亚磺酰基烷基和烯基，尚未通过生化研究解决。在这项研究中，通过 Brassica drepanensis 和 B. atlantica 回交群体的遗传分析，对两个假设模型进行了测试。结果支持其中一个模型，即 3-甲基硫代丙基葡萄糖苷通过两个位点的显性等位基因的作用依次转化为 3-甲基亚磺酰基丙基，然后转化为 2-丙烯基葡萄糖苷。RFLP 图谱将两个位点定位在与 B. napus N19 连锁群同源的同一连锁群上。讨论了这些结果对于遗传操纵 Brassica 中硫代葡萄糖苷以改善风味和营养价值，以及研究植物-昆虫相互作用的意义。
