Alam R, Rozniecki J, Kuzminska B
Ann Allergy. 1985 Dec;55(6):825-9.
Peripheral blood lymphocytes from atopic asthmatic patients cultured overnight (16 hours) produced a novel lymphokine-histamine releasing factor (HRF) in the culture supernatant. Activity of this lymphokine has been tested in the basophil histamine release test. Supernatant from unstimulated lymphocytes released 29% histamine from basophils. A short incubation of lymphocytes with skin test-positive allergens (grass, mite) enhanced the generation of HRF. Skin test-negative allergens had no effect. HRF appeared in the supernatant as early as four hours after the start of the culture and its production level remained high even at 48 hours. Gel-filtration with Sephadex G-75 revealed an apparent molecular weight for HRF in the range of 10,000 to 15,000. HRF is relatively heat stable at 56 degrees C.
来自特应性哮喘患者的外周血淋巴细胞培养过夜（16小时）后，在培养上清液中产生了一种新型淋巴因子——组胺释放因子（HRF）。已在嗜碱性粒细胞组胺释放试验中测试了这种淋巴因子的活性。未刺激淋巴细胞的上清液使嗜碱性粒细胞释放29%的组胺。淋巴细胞与皮肤试验阳性变应原（草、螨）短暂孵育可增强HRF的产生。皮肤试验阴性的变应原则无影响。HRF最早在培养开始后4小时出现在上清液中，其产生水平即使在48小时时仍保持较高。用葡聚糖凝胶G-75进行凝胶过滤显示，HRF的表观分子量在10,000至15,000范围内。HRF在56℃时相对耐热。
