Alam R, Grant J A, Lett-Brown M A
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550.
J Clin Invest. 1988 Dec;82(6):2056-62. doi: 10.1172/JCI113826.
Human mononuclear cells (MNC) secrete histamine-releasing factor(s) (HRF) when cultured in vitro. HRF induces the release of histamine and other mediators from basophils and mast cells. We have shown that MNC also produced a histamine release inhibitory factor (HRIF), and that the synthesis is augmented by culture with physiologic concentrations of histamine (10(-10) to 10(-6) M) and by the mitogen concanavalin A (Con A). HRIF does not affect release initiated by other secretagogues such as allergen, anti-IgE, C5a, Con A, and phorbol myristate acetate. HRIF requires a preincubation with the cells for 5-10 min for maximal inhibition, and this effect is not abolished by washing the cells after the preincubation. The biological activity of HRIF is protease-sensitive, neuraminidase-resistant, and relatively heat-stable. HRIF can be distinguished from HRF by a lower apparent molecular mass (8,000-10,000 D).
人单核细胞（MNC）在体外培养时会分泌组胺释放因子（HRF）。HRF可诱导嗜碱性粒细胞和肥大细胞释放组胺及其他介质。我们已经表明，MNC还产生组胺释放抑制因子（HRIF），并且通过与生理浓度的组胺（10⁻¹⁰至10⁻⁶M）以及丝裂原伴刀豆球蛋白A（Con A）共同培养可增强其合成。HRIF不影响由其他促分泌剂引发的释放，如变应原、抗IgE、C5a、Con A和佛波酯肉豆蔻酸酯乙酸酯。HRIF需要与细胞预孵育5 - 10分钟以达到最大抑制效果，并且这种效果在预孵育后洗涤细胞时不会被消除。HRIF的生物活性对蛋白酶敏感，对神经氨酸酶有抗性，并且相对耐热。HRIF可以通过较低的表观分子量（8000 - 10000 D）与HRF区分开来。
