Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, New York, USA.
Theor Appl Genet. 1996 Feb;92(2):230-44. doi: 10.1007/BF00223380.
To detect QTLs controlling traits of agronomic importance in rice, two elite homozygous lines 9024 and LH422, which represent the indica and japonica subspecies of rice (Oryza sativa), were crossed. Subsequently a modified single-seed-descent procedure was employed to produce 194 recombinant inbred lines (F8). The 194 lines were genotyped at 141 RFLP marker loci and evaluated in a field trial for 13 quantitative traits including grain yield. Transgressive segregants were observed for all traits examined. The number of significant QTLs (LOD [Symbol: see text] 2.0) detected affecting each trait ranged from one to six. The percentage of phenotypic variance explained by each QTL ranged from 5.1% to 73.7%. For those traits for which two or more QTLs were detected, increases in the traits were conditioned by indica alleles at some QTLs Japonica alleles at others. No significant evidence was found for epistasis between markers associated with QTLs and all the other markers. Pleitropic effects of single QTLs on different traits are suggested by the observation of clustering of QTLs. No QTL for traits was found to map to the vicinity of major gene loci governing the same traits qualitatively. Evidence for putative orthologous QTLs across rice, maize, oat, and barley is discussed.
为了在水稻中检测控制农艺性状的 QTL，我们将代表籼稻和粳稻亚种的两个优良纯合系 9024 和 LH422 进行杂交。随后，采用改良的单粒传代程序产生了 194 个重组自交系（F8）。这 194 个系在 141 个 RFLP 标记基因座进行了基因型分析，并在田间试验中对包括产量在内的 13 个数量性状进行了评估。所有检查的性状都观察到了超亲分离。检测到影响每个性状的显著 QTL（LOD [符号：见正文] 2.0）的数量从 1 到 6 个不等。每个 QTL 解释的表型方差比例从 5.1%到 73.7%不等。对于那些检测到两个或更多 QTL 的性状，一些 QTL 上的籼稻等位基因和其他 QTL 上的粳稻等位基因决定了性状的增加。在与 QTL 相关的标记和所有其他标记之间没有发现明显的上位性证据。单个 QTL 对不同性状的多效性效应是通过观察 QTL 的聚类得出的。没有发现任何 QTL 与控制同一性状的主基因座附近的性状相关联。讨论了在水稻、玉米、燕麦和大麦中潜在的同源 QTL 的证据。
