INRA, Station d'Amélioration des Plantes, Domaine de Crouelle, 63039, Clermont-Ferrand Cedex, France.
Theor Appl Genet. 1996 Mar;92(3-4):340-6. doi: 10.1007/BF00223677.
Wheat storage proteins were evaluated by SDS-PAGE in a population of 206 doubled haploid (DH) lines, produced from a cross between bread wheat cvs Chinese Spring (CS) and Courtot (CT). The analysis of gliadins and high- and low-molecular-weight glutenins gave rise to 11 protein markers between parental varieties. Among these, one each was encoded at the Glu-A1, Gli-A1, Gli-A2, Gli-A5, Glu-B3, Gli-B1 and Gli-D1 loci and four were encoded at the Glu-D3 locus. Only the Gli-A2 marker showed a distorted segregation. A distance of 1.94 cM was evaluated between the Gli-A1 locus and the recently found Gli-A5 locus. Among the DH lines, only nine exhibited an unexpected pattern. The chromosome allocation was determined for almost all the LMW-GS and gliadin bands of CS using nullitetrasomic and ditelosomic lines. Two C LMW-GS were found to be coded by 6DS. Similarly, substitution lines into CT allowed the allelic determination of numerous LMW-GS and gliadin bands. A correspondence between gliadin markers separated in SDS-PAGE and in A-PAGE revealed that the common allele Gli-Aa between CS and CT determined in A-PAGE was able to be separated into two alleles when SDS-PAGE was used.
采用 SDS-PAGE 对来自普通小麦品种 Chinese Spring（CS）和 Courtot（CT）杂交的 206 个加倍单倍体（DH）品系的贮藏蛋白进行了评价。醇溶蛋白和高分子量及低分子量谷蛋白分析共产生了 11 个在双亲品种间的蛋白标记，其中 Glu-A1、Gli-A1、Gli-A2、Gli-A5、Glu-B3、Gli-B1 和 Gli-D1 座位各有 1 个，Glu-D3 座位有 4 个。仅 Gli-A2 标记显示分离偏倚。Gli-A1 座位和最近发现的 Gli-A5 座位之间的距离为 1.94cM。在 DH 系中，只有 9 个系显示出意外的模式。利用单体附加系和缺体二体系确定了 CS 几乎所有 LMW-GS 和醇溶蛋白带的染色体分配。发现 2 个 C 型 LMW-GS 由 6DS 编码。同样，CT 的代换系也允许对许多 LMW-GS 和醇溶蛋白带进行等位基因鉴定。在 SDS-PAGE 和 A-PAGE 中分离的醇溶蛋白标记之间存在对应关系，表明在 A-PAGE 中确定的 CS 和 CT 之间的共同等位基因 Gli-Aa 能够在 SDS-PAGE 中分为两个等位基因。
