Warawa Jonathan M, Lawrenz Matthew B
Department of Microbiology and Immunology, Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases, University of Louisville School of Medicine, Louisville, KY, USA.
Methods Mol Biol. 2014;1098:169-81. doi: 10.1007/978-1-62703-718-1_14.
Diagnostic imaging is a powerful tool that has recently been applied towards the study of infectious diseases. Optical imaging of bioluminescently labeled bacteria in infected animals allows for real-time analysis of bacterial proliferation and dissemination during infection without sacrificing the animal. Imaging also allows for tracking of disease progression in an individual subject over time, has the potential to reveal previously overlooked sites of infection, and reduces the number of research animals used in pathogenesis studies. Here, we describe the use of a deep-cooled CCD camera imager to record light emitted from bacteria during infection. We also describe the process of correlating bioluminescence to bacterial numbers by ex vivo imaging of necropsied tissues. Together these techniques can be used to estimate bacterial burdens in host tissues both in vivo and ex vivo using bioluminescent imaging.
诊断成像技术是一种强大的工具，最近已被应用于传染病研究。对感染动物体内经生物发光标记的细菌进行光学成像，能够在不牺牲动物的情况下实时分析感染过程中细菌的增殖和扩散情况。成像技术还能跟踪个体受试者疾病随时间的进展，有可能揭示以前被忽视的感染部位，并减少发病机制研究中所用实验动物的数量。在此，我们描述了使用深度冷却的电荷耦合器件（CCD）相机成像仪来记录感染过程中细菌发出的光。我们还描述了通过对尸检组织进行离体成像，将生物发光与细菌数量相关联的过程。综合起来，这些技术可用于通过生物发光成像在体内和离体状态下估计宿主组织中的细菌载量。
