State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, 100101, Beijing, China.
Theor Appl Genet. 1996 Jun;92(8):1103-7. doi: 10.1007/BF00224056.
The Hexaploid wheat variety Fukuho was crossed with Tripsacum dactyloides (2n=4x=72). The total fertilization frequencies for the egg cell, polar nuclei, and both, were 58.3%, 26.8% and 58.9% of the 168 ovaries examined. However, the fertilization frequency of single polar nuclei was much lower at only 0.6%. The total frequency of fertilization was higher than that in wheat x maize crosses. A total of 49 hexaploid wheat varieties, including Hope carrying the dominant genes Kr1 and Kr2, were crossed with T. dactyloides, and most gave embryos. The embryoformation frequencies ranged from 0.5% to 59.0%. A higher frequency of 32.0% embryo formation was obtained following pollination of the variety Hope. In comparison with embryo formation in wheat x maize crosses the difference of embryo-formation frequencies between the two crosses was significant. The results of high frequencies of fertilization and embryo formation in wheat x T. dactyloides crosses indicated that the Kr genes are as inactive in wheat x T. dactyloides, as they are in wheat x maize crosses, and also that the efficiency of fertilization and embryo formation is higher in wheat x T. dactyloides than in what x maize crosses. The potential of wheat x T. dactyloides crosses for wheat haploid production and wheat improvement is discussed.
六倍体小麦品种福光与节节麦（2n=4x=72）杂交。在 168 个被观察的胚珠中，卵细胞、极核和二者的总受精频率分别为 58.3%、26.8%和 58.9%。然而，单个极核的受精频率要低得多，仅为 0.6%。总的受精频率高于小麦与玉米的杂交。总共 49 个六倍体小麦品种，包括携带显性基因 Kr1 和 Kr2 的 Hope，与节节麦杂交，大多数都产生了胚胎。胚胎形成频率从 0.5%到 59.0%不等。在 Hope 品种授粉后，胚胎形成频率达到 32.0%的较高水平。与小麦与玉米的杂交相比，这两种杂交的胚胎形成频率差异显著。小麦与节节麦杂交中高受精率和胚胎形成频率的结果表明，Kr 基因在小麦与节节麦杂交中与在小麦与玉米杂交中一样不活跃，并且小麦与节节麦的受精效率和胚胎形成效率高于小麦与玉米的杂交。讨论了小麦与节节麦杂交在小麦单倍体生产和小麦改良方面的潜力。
