Park Su-Young, Neupane Ganesh Prasad, Lee Sung Ok, Lee Jong Suk, Kim Mi-Young, Kim Sun Yeou, Park Byung Chul, Park Young-Joon, Kim Jung-Ae
College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
Arch Pharm Res. 2014 Feb;37(2):253-62. doi: 10.1007/s12272-013-0260-x. Epub 2013 Oct 30.
In inflammatory bowel disease (IBD), colon epithelial cells express a variety of inflammatory mediators, including chemokines, which perpetuate inflammatory response. In the current study, we report that water extract of Pogostemon cablin Bentham aerial parts (PCW), which has traditionally been used for treatment of the common cold and infectious disease, suppressed colon inflammation. Treatment with PCW resulted in effective inhibition of tumor necrosis factor (TNF)-α-induced adhesion of monocytes to HT-29 human colonic epithelial cells. In a trinitrobenzene sulfonic acid (TNBS)-induced rat model of IBD, PCW suppressed clinical signs of colitis, including weight loss, colon tissue myeloperoxidase activity, a marker for inflammatory cell infiltration, and cyclooxygenase-2 expression in a dose-dependent manner. In addition, PCW suppressed TNBS-induced mRNA expression of IL-8, MCP-1, and IL-6 in rat colon. The nuclear level of NF-κB in TNBS-treated rat colon and NF-κB luciferase reporter gene activity in TNF-α-treated HT-29 cells were significantly inhibited by PCW. Taken together, the results of this study suggest that PCW suppressed colon inflammation via suppression of NF-κB-dependent expression of pro-inflammatory cytokines.
在炎症性肠病（IBD）中，结肠上皮细胞表达多种炎症介质，包括趋化因子，这些介质会使炎症反应持续存在。在本研究中，我们报告称，广藿香地上部分的水提取物（PCW），传统上用于治疗感冒和传染病，可抑制结肠炎症。用PCW处理可有效抑制肿瘤坏死因子（TNF）-α诱导的单核细胞与HT-29人结肠上皮细胞的黏附。在三硝基苯磺酸（TNBS）诱导的IBD大鼠模型中，PCW以剂量依赖的方式抑制结肠炎的临床症状，包括体重减轻、结肠组织髓过氧化物酶活性（炎症细胞浸润的标志物）和环氧合酶-2表达。此外，PCW抑制TNBS诱导的大鼠结肠中IL-8、MCP-1和IL-6的mRNA表达。PCW显著抑制TNBS处理的大鼠结肠中NF-κB的核水平以及TNF-α处理的HT-29细胞中NF-κB荧光素酶报告基因活性。综上所述，本研究结果表明，PCW通过抑制NF-κB依赖的促炎细胞因子表达来抑制结肠炎症。
