Kraaijeveld C A, Kamphuis W, Benaissa-Trouw B J, Harmsen M, Snippe H
Int Arch Allergy Appl Immunol. 1986;79(1):86-9. doi: 10.1159/000233948.
The modulation by the interferon (IFN) inducers poly I:C and Newcastle disease virus (NDV) of the effector phase of adjuvant-enhanced delayed-type hypersensitivity (DH) was studied in mice. A strongly enhanced DH was induced in mice to ultraviolet (UV) light inactivated Semliki forest virus (SFV) by the use of the adjuvant dimethyldioctadecylammonium bromide. At day 6 after intracutaneous immunization, DH was elicited with SFV and measured 24 and 48 h later as increase in footpad thickness (footpad swelling test). Systemic, intravenous administration of either poly I:C, UV-inactivated NDV, or NDV-induced IFN prior to elicitation of DH with antigen resulted in a temporarily suppressed DH reaction. Both the poor swelling at 3 h and strong swelling at 24 h were suppressed, while the swelling at 48 h was enhanced. The model described provides a sensitive in vivo method to study modulating effects of drugs and microbial agents on the effector phase of DH.
在小鼠中研究了干扰素（IFN）诱导剂聚肌胞苷酸（poly I:C）和新城疫病毒（NDV）对佐剂增强的迟发型超敏反应（DH）效应阶段的调节作用。通过使用佐剂二甲基二十八烷基溴化铵，在小鼠中诱导出对紫外线（UV）灭活的Semliki森林病毒（SFV）的强烈增强的DH。在皮内免疫后第6天，用SFV引发DH，并在24小时和48小时后通过足垫厚度增加（足垫肿胀试验）来测量。在用抗原引发DH之前，全身性静脉注射poly I:C、紫外线灭活的NDV或NDV诱导的IFN，会导致DH反应暂时受到抑制。3小时时的轻微肿胀和24小时时的强烈肿胀均受到抑制，而48小时时的肿胀增强。所描述的模型提供了一种灵敏的体内方法，用于研究药物和微生物制剂对DH效应阶段的调节作用。
