Huitfeldt H S, Brandtzaeg P
Histochemistry. 1985;83(5):381-9. doi: 10.1007/BF00509196.
Various polyclonal and monoclonal antibodies to keratins were used to stain different human muscle tissues by paired immunofluorescence and the unlabelled antibody peroxidase-anti-peroxidase method. In the myocardium, distinct coloration of the intercalated discs was produced by two polyclonal reagents to human epidermal keratins but not by two monoclonal antibodies to cytokeratins from pig renal tubular cells. In the myometrium--mainly in the middle layer of the uterine wall--cytoplasmic coloration of a varying fraction of the smooth muscle bundles was produced, especially by one of the polyclonal and by both monoclonal reagents. The staining was often confined to the perinuclear region. The keratin-positive myometrial cells usually coexpressed vimentin and actin in various proportions. These findings indicated that intermediate filaments of the keratin type, or antigenically similar elements, are not restricted to cells of epithelial origin. Other types of muscle cells did not react with keratin antibodies, but keratin-positive macrophages were occasionally found in tongue musculature and in inflamed epicardium. Altogether, our observations emphasize that keratin reactivity cannot be considered specific for epithelial (or mesothelial) cells without reservation.
运用多种针对角蛋白的多克隆抗体和单克隆抗体，通过配对免疫荧光法以及未标记抗体过氧化物酶-抗过氧化物酶法对不同的人体肌肉组织进行染色。在心肌中，两种针对人表皮角蛋白的多克隆试剂能使闰盘产生明显的着色，但两种针对猪肾小管细胞细胞角蛋白的单克隆抗体则不能。在子宫肌层——主要是子宫壁的中层——不同比例的平滑肌束出现了细胞质着色，尤其是其中一种多克隆抗体和两种单克隆试剂所致。染色通常局限于核周区域。角蛋白阳性的子宫肌层细胞通常以不同比例共表达波形蛋白和肌动蛋白。这些发现表明，角蛋白类型的中间丝或抗原性相似的成分并不局限于上皮来源的细胞。其他类型的肌肉细胞对角蛋白抗体无反应，但在舌肌组织和发炎的心外膜中偶尔能发现角蛋白阳性的巨噬细胞。总之，我们的观察结果强调，不能毫无保留地认为角蛋白反应性是上皮（或间皮）细胞所特有的。
