National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China.
Yu'An Institute of Wheat, Wen County, Jiaozuo, 454850, China.
Sci Rep. 2020 Mar 16;10(1):4801. doi: 10.1038/s41598-020-61888-1.
Agronomic characteristics and tolerance to biotic and abiotic stresses in hexaploid wheat can be drastically improved through wheat-alien introgression. However, the transcriptional level interactions of introduced alien genes in the wheat genetic background is rarely investigated. In this study, we report the genome-wide impacts of introgressed chromosomes derived from Ae. longissima on gene transcriptions of the wheat landrace Chinese Spring. RNA-seq analyses demonstrated 5.37% and 4.30% of the genes were significantly differentially expressed (DEGs) in CS-Ae. longissima disomic 3S#2(3B) substitution line TA3575 and disomic 6S#3 addition line TA7548, respectively when compared to CS. In addition, 561 DEGs, including 413 up-regulated and 148 down-regulated or not transcribed genes, were simultaneously impacted by introgressed chromosomes 3S#2 and 6S#3, which accounts for 41.25% of the DEGs in TA3575 and 38.79% in TA7548. Seventeen DEGs, annotated as R genes, were shared by both introgression lines carrying chromosomes 3S#2 and 6S#3, which confer resistance to powdery mildew. This study will benefit the understanding of the wheat gene responses as result of alien gene(s) or chromosome intogression and the plant defense response initiated by powdery mildew resistance genes in chromosomes 3S#2 and 6S#3.
通过小麦外源基因导入，可极大地改善六倍体小麦的农艺性状和生物及非生物胁迫耐受性。然而，外源基因在小麦遗传背景中的转录水平相互作用却很少被研究。本研究报告了来自长穗偃麦草的导入染色体对小麦地方品种春小麦基因转录的全基因组影响。RNA-seq 分析表明，与春小麦相比，在 TA3575（3B 染色体的二体代换系）和 TA7548（6S#3 染色体的二体附加系）中，分别有 5.37%和 4.30%的基因显著差异表达（DEGs）。此外，同时受到 3S#2 和 6S#3 导入染色体影响的差异表达基因有 561 个，包括 413 个上调和 148 个下调或未转录基因，占 TA3575 中 DEGs 的 41.25%和 TA7548 中的 38.79%。17 个注释为 R 基因的 DEGs 在携带 3S#2 和 6S#3 染色体的两个导入系中共享，这些基因赋予了对白粉病的抗性。本研究将有助于理解由于外源基因或染色体导入引起的小麦基因响应以及 3S#2 和 6S#3 染色体上白粉病抗性基因引发的植物防御反应。
