Research Institute of Agricultural Resources, Ishikawa Agricultural College, Ishikawa 921, Nonoichi-machi, Japan.
Theor Appl Genet. 1995 Nov;91(6-7):830-5. doi: 10.1007/BF00223888.
Random amplified polymorphic DNA (RAPD) markers were utilized for the identification of Lilium species and inter-specific hybrids. The optimum annealing temperature of the polymerase chain reaction (PCR) for the RAPD assay in Lilium was 54 °C, which is relatively higher than the temperature used for other genera reported by previous researchers. Among 76 primers used to amplify genomic DNA by PCR, 18 primers (24%) generated polymorphic DNA fragments in Lilium species and hybrids. Cultivars were also identified by RAPD markers. Some amplified fragments were unique to species of each section and to hybrids derived from these species; that is, they were the section-specific DNA markers. Sections, Sinomartagon, Leucolirion b, Leucolirion a and Archelirion could be identified by 6 section-specific markers amplified with five primers. Seven inter-section hybrids showed the section-specific bands of both parental sections, indicating that these markers would be useful for identifying the parental sections of inter-section hybrids.
随机扩增多态性 DNA（RAPD）标记被用于鉴定百合属物种和种间杂种。在百合属中进行 RAPD 分析的聚合酶链反应（PCR）的最佳退火温度为 54°C，这比以前研究人员报道的其他属的温度要高。在用于扩增基因组 DNA 的 76 个引物中，18 个引物（24%）在百合属物种和杂种中产生了多态性 DNA 片段。RAPD 标记也可用于鉴定品种。一些扩增片段是每个部分的特有物种和这些物种衍生的杂种所特有；也就是说，它们是特有部分的 DNA 标记。百合属的岷江百合亚属、无斑百合亚属、鹿葱亚属和钟花组可通过用 5 个引物扩增的 6 个特有部分标记来识别。7 个组间杂种显示了双亲组的特有带，表明这些标记可用于鉴定组间杂种的双亲组。
