Institute of Agronomy and Plant Breeding, University of Göttingen, Von-Siebold-Strasse 8, D-37075, Göttingen, Germany.
Theor Appl Genet. 1995 Nov;91(6-7):972-7. doi: 10.1007/BF00223908.
A F1 microspore-derived DH population, previously used for the development of a rapeseed RFLP map, was analysed for the distribution of erucic acid and seed oil content. A clear three-class segregation for erucic acid content could be observed and the two erucic acid genes of rapeseed were mapped to two different linkage groups on the RFLP map. Although the parents of the segregating DH population showed no significant difference in seed oil content, in the DH population a transgressive segregation in oil content was observed. The segregation closely followed a normal distribution, characteristic of a quantitative trait. Using the program MAPMAKER/QTL, three QTLs for seed oil content could be mapped on three different linkage groups. The additive effects of these QTLs explain about 51% of the phenotypic variation observed for this trait in the DH population. Two of the QTLs for oil content showed a close association in location to the two erucic acid genes, indicating a direct effect of the erucic acid genes on oil content.
一个 F1 花粉衍生的 DH 群体，先前被用于油菜的 RFLP 图谱的发展，被分析了油酸和油含量的分布。可以观察到油酸含量的明显的三类别分离，并且油菜的两个油酸基因被映射到 RFLP 图谱上的两个不同的连锁群上。尽管分离的 DH 群体的亲本在种子油含量上没有显著差异，但在 DH 群体中观察到油含量的超亲分离。这种分离紧密遵循正态分布，是一种数量性状的特征。使用 MAPMAKER/QTL 程序，可以将三个与种子油含量有关的 QTL 映射到三个不同的连锁群上。这些 QTL 的加性效应解释了 DH 群体中观察到的这个性状的表型变异的约 51%。两个油含量的 QTL 与两个油酸基因在位置上有密切的关联，表明油酸基因对油含量有直接的影响。
