New Zealand Institute for Crop and Food Research Limited, Private Bag 4704, Christchurch, New Zealand.
Theor Appl Genet. 1995 Dec;91(8):1288-92. doi: 10.1007/BF00220943.
Hordeum bulbosum L. is a source of disease resistance genes that would be worthwhile transferring to barley (H. vulgare L.). To achieve this objective, selfed seed from a tetraploid H. vulgare x H. bulbosum hybrid was irradiated. Subsequently, a powdery mildew-resistant selection of barley phenotype (81882/83) was identified among field-grown progeny. Using molecular analyses, we have established that the H. bulbosum DNA containing the powdery mildew resistance gene had been introgressed into 81882/83 and is located on chromosome 2 (2I). Resistant plants have been backcrossed to barley to remove the adverse effects of a linked factor conditioning triploid seed formation, but there remains an association between powdery mildew resistance and non-pathogenic necrotic leaf blotching. The dominant resistance gene is allelic to a gene transferred from H. bulbosum by co-workers in Germany, but non-allelic to all other known powdery mildew resistance genes in barley. We propose Mlhb as a gene symbol for this resistance.
大麦属(Hordeum vulgare L.)是一种抗病基因的来源,值得将其转移到大麦属(Hordeum vulgare L.)中。为了实现这一目标,对四倍体大麦属(Hordeum vulgare L.)×大麦属(Hordeum bulbosum L.)杂种的自交种子进行了辐射处理。随后,在田间生长的后代中鉴定出了大麦属(Hordeum vulgare L.)表型的抗白粉病选择(81882/83)。通过分子分析,我们已经确定,含有白粉病抗性基因的大麦属(Hordeum bulbosum L.)DNA已被导入 81882/83,并位于染色体 2(2I)上。抗性植株已回交至大麦属(Hordeum vulgare L.),以去除导致三倍体种子形成的连锁因子的不利影响,但白粉病抗性与非致病性坏死叶斑之间仍存在关联。显性抗性基因与德国同事从大麦属(Hordeum bulbosum L.)转移的基因等位,但与大麦属(Hordeum vulgare L.)中所有其他已知的白粉病抗性基因均非同位。我们建议将 Mlhb 作为该抗性的基因符号。