School of Natural and Environmental Sciences, Ridley Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Department of Chemical Engineering, Biotechnology and Materials, Centre for Biotechnology and Bioengineering (CeBiB), University of Chile, Beauchef 851, Santiago, Chile.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1863-1874. doi: 10.1007/s10482-019-01314-3. Epub 2019 Aug 12.
A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
发现一组寡核苷酸引物 Rubro223f 和 Rubro454r 可扩增 Rubrobacter 型菌株 16S rRNA 基因的 267 个核苷酸序列。这些引物可将该属成员与其他深植根的放线菌谱系区分开来，这些谱系对应于Thermoleophilia 纲中的属 Conexibacter、Gaiella、Parviterribacter、Patulibacter、Solirubrobacter 和 Thermoleophilum。从阿塔卡马沙漠两个地点采集的土壤样本中提取的 DNA 进行扩增，可产生约 267 个核苷酸的 DNA 带。从这些条带制备的 DNA 文库的测序表明，所有克隆都属于 Rubrobacter 属的进化辐射范围内。大多数克隆被分配到两个谱系，这两个谱系与由 Rubrobacter 型菌株组成的系统发育谱系分开。可以得出结论，引物 Rubro223f 和 Rubro454r 是 Rubrobacter 属的特异性引物，可用于检测 Atacama 沙漠和其他生物群落中该属成员的存在和丰度。
