Zamani Mehrangiz, Goodarzi Mohammad Taghi, Lavasani Navaz Sadat, Khosravi Abas
Department of Obstetrics and Gynecology, Hamadan University of Medical Sciences, Hamadan, Iran;
Iran J Med Sci. 2013 Sep;38(3):227-32.
Vitamin C is a water-soluble antioxidant that not only stimulates and protects collagen synthesis but also plays an important role in maintaining cellular integrity in a normal pregnancy. This study surveyed the effects of ascorbic acid on the serum level of unconjugated estriol and the relationship between unconjugated estriol and preterm premature rupture of membrane (PPROM).
This double-blind, randomized clinical trial recruited 60 patients with predisposing factors to PPROM. The women were randomly divided into two groups of intervention and control and received vitamin C and placebo, respectively. The intervention group received 250 mg vitamin C twice a day and the controls received the placebo only. Unconjugated estriol was measured using the ELISA. All data were extracted and recorded in a checklist and compared using descriptive statistics as well as the x (2), Fisher exact, and t tests.
The demographic data showed no difference between the two groups. The mean level of serum unconjugated estriol was significantly lower in the intervention group than in the control group (P=0.044). Also, the frequency of PPROM was lower in the intervention group, but the difference was not significant (P>0.05). Unconjugated estriol levels were not significantly different between the healthy women and the PPROM patients.
This study demonstrated that vitamin C administration decreased unconjugated estriol levels in the patients with PPROM. The findings of this study also indicated that administration of ascorbic acid was a safe and effective method to reduce the incidence of PPROM. Alteration in unconjugated estriol is an active mediator for this effect.
维生素C是一种水溶性抗氧化剂，不仅能刺激和保护胶原蛋白的合成，还在维持正常妊娠时的细胞完整性方面发挥重要作用。本研究调查了抗坏血酸对血清未结合雌三醇水平的影响以及未结合雌三醇与胎膜早破（PPROM）之间的关系。
这项双盲、随机临床试验招募了60名有PPROM易感因素的患者。这些女性被随机分为干预组和对照组，分别接受维生素C和安慰剂。干预组每天两次服用250毫克维生素C，对照组仅接受安慰剂。使用酶联免疫吸附测定法（ELISA）测量未结合雌三醇。所有数据均提取并记录在一份清单中，并使用描述性统计以及卡方检验、Fisher精确检验和t检验进行比较。
人口统计学数据显示两组之间无差异。干预组血清未结合雌三醇的平均水平显著低于对照组（P = 0.044）。此外，干预组PPROM的发生率较低，但差异不显著（P>0.05）。健康女性和PPROM患者之间未结合雌三醇水平无显著差异。
本研究表明，给予维生素C可降低PPROM患者的未结合雌三醇水平。本研究结果还表明，给予抗坏血酸是降低PPROM发生率的一种安全有效的方法。未结合雌三醇的改变是这种作用的一个活性介质。
