Department of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, China.
Biomed Res Int. 2013;2013:146953. doi: 10.1155/2013/146953. Epub 2013 Sep 23.
In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.
在组织工程领域，具有高生物相容性的聚合物材料，如聚乳酸和聚乙醇酸，已被广泛用于构建活组织。对于下咽组织工程，骨骼肌是整个器官的一个重要功能部分，它组装成单向排列的肌管。在这项研究中，使用具有微通道图案的聚氨酯（PU）支架为接种的人成肌细胞提供定向引导。由于 PU 的低亲水性，支架接枝丝素蛋白（PU-SF）或明胶（PU-Gel）以改善其细胞黏附性能。支架随时间缓慢降解，通过表面接枝提高了其机械性能和亲水性。此外，与对照组或 PU-Gel 相比，接种在 PU-SF 上的成肌细胞具有更高的增殖率和更好的分化能力。我们的结果表明，接种有成肌细胞的聚氨酯支架有望指导下咽肌肉再生。
