Department of Biotechnology, Punjabi University, Punjab, Patiala 147 002, India.
Biomed Res Int. 2013;2013:583850. doi: 10.1155/2013/583850. Epub 2013 Sep 24.
The present study aims to investigate the probiotic nature of Pediococcus acidilactici MTCC5101 by an in vitro assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (GI) tract using Caco-2 cell line. Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers. The effect on wellness was assessed by studying blood biochemical parameters of the volunteers. The survival of the bacteria was assessed using PCR-based detection of P. acidilactici MTCC5101 in fecal samples. The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations. Oral feeding study for assessing the survival of bacteria in GI tract of volunteers showed the strain to be established in the GI tract which survived for about 2 weeks after feeding.
本研究旨在通过体外检测 Caco-2 细胞系中肠道上皮细胞对人胃肠道 (GI) 道的细菌黏附作用，来研究植物乳杆菌 MTCC5101 的益生菌特性。为了评估其在 GI 道中的体内存活情况，我们在 10 名健康志愿者的帮助下进行了口服喂养。通过研究志愿者的血液生化参数来评估其对健康的影响。使用基于 PCR 的方法检测粪便样本中的 P. acidilactici MTCC5101 来评估细菌的存活情况。体外 SEM 观察结果表明，P. acidilactici MTCC5101 能够黏附在肠道上皮 Caco-2 细胞系上，这增强了其益生菌特性。为了评估细菌在志愿者 GI 道中的存活情况而进行的口服喂养研究表明，该菌株在 GI 道中定植，在喂养后约 2 周仍能存活。
