活的和热灭活的TAP041的益生菌和免疫刺激作用
Probiotic and immunostimulating effects of live and heat-killed TAP041.
作者信息
Lee Chaeeun, Park Young-Seo
机构信息
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
出版信息
Food Sci Biotechnol. 2024 Feb 19;33(9):2189-2200. doi: 10.1007/s10068-024-01530-2. eCollection 2024 Jul.
UNLABELLED
The selected strain, TAP041, showing an excellent ability to reduce the glyoxal and methylglyoxal levels, was identified by 16S rRNA gene-based phylogenetic analysis as . It demonstrated probiotic properties, including acid, bile salt, pancreatin, lysozyme tolerance, gut adhesion, and auto/coaggregation. In RAW264.7 macrophages, both live and heat-killed strains induced nitric oxide production and activated inducible nitric oxide synthase. RAW264.7 treated with TAP041 increased the expression level of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and cyclooxygenase-2, and regulated the expression of c-Jun amino-terminal kinase, p38, and extracellular signal-regulated kinase. These findings suggest that both live and heat-killed TAP041 can be used as potential immunostimulatory agents in functional food additives.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-024-01530-2.
未标记
所选菌株TAP041具有出色的降低乙二醛和甲基乙二醛水平的能力，通过基于16S rRNA基因的系统发育分析鉴定为 。它表现出益生菌特性，包括耐酸、耐胆盐、耐胰蛋白酶、耐溶菌酶、肠道黏附以及自聚集/共聚集。在RAW264.7巨噬细胞中，活菌株和热灭活菌株均诱导一氧化氮产生并激活诱导型一氧化氮合酶。用TAP041处理的RAW264.7增加了肿瘤坏死因子-α、白细胞介素（IL）-1β、IL-6和环氧化酶-2的表达水平，并调节了c-Jun氨基末端激酶、p38和细胞外信号调节激酶的表达。这些发现表明，活的和热灭活的TAP041均可作为功能性食品添加剂中的潜在免疫刺激剂。
补充信息
在线版本包含可在10.1007/s10068-024-01530-2获取的补充材料。
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