1 Department of Food and Biotechnology, Korea University , Seoul, Republic of Korea.
J Med Food. 2013 Nov;16(11):1057-61. doi: 10.1089/jmf.2012.0280. Epub 2013 Oct 31.
In this study, the protective effects of Brussels sprouts extract and its major constituents against oxidative stress-induced damages were investigated in rat pheochromocytoma cells and Institute of Cancer Research mice. The major constituents of Brussels sprouts (3,4',5,7-tetrahydroxyflavone (kaempferol), indole-3-carbinol, and phenethyl isothiocyanate) were selectively tested. Of these, the flavonoid compound, kaempferol exhibited the highest potency in radical scavenging activity (1,1-diphenyl-2-picryl hydrazyl assay and oxygen radical absorbance capacity assay) and was most protective against oxidative stress in neuronal cell assays (measurement of intracellular oxidative stress levels and cell viability). In mice, after 4 weeks of kaempferol administration, significant protection against amyloid beta (Aβ) peptide-induced neurotoxicity was also observed, as assessed through the passive avoidance test. Taken together, the results suggest that Brussels sprouts could be protective against Aβ-induced neurotoxicity, possibly due to the antioxidative capacity of its major constituent, kaempferol.
在这项研究中，研究人员调查了抱子甘蓝提取物及其主要成分对大鼠嗜铬细胞瘤细胞和癌症研究所小鼠氧化应激损伤的保护作用。研究人员对抱子甘蓝的主要成分（3,4',5,7-四羟基黄酮（山奈酚）、吲哚-3-甲醇和苯乙基异硫氰酸酯）进行了选择性测试。其中，黄酮类化合物山奈酚在清除自由基活性（1,1-二苯基-2-苦基肼法和氧自由基吸收能力测定法）和神经元细胞检测中的抗氧化应激方面表现出最高的效力（测量细胞内氧化应激水平和细胞活力）。在小鼠中，经过 4 周的山奈酚给药后，通过被动回避试验观察到对淀粉样β（Aβ）肽诱导的神经毒性也有显著的保护作用。总的来说，这些结果表明，抱子甘蓝可能具有对抗 Aβ 诱导的神经毒性的作用，这可能是由于其主要成分山奈酚的抗氧化能力。
