Department of Food and Biotechnology, Korea University, Seoul, South Korea.
J Med Food. 2020 May;23(5):523-534. doi: 10.1089/jmf.2019.4563. Epub 2020 Mar 2.
A variety of natural compounds have been used to reduce the oxidative stress associated with Alzheimer's disease, and many of these defend cells from oxidative stress-induced neuronal toxicity. In this study, the protective effect of radish () extract was investigated in mice and PC12 cells. behavioral tests were completed to examine the protective effects of the extract on amyloid beta (A)-peptide-induced learning and memory impairment in a mouse model. The extract increased spontaneous alternation behaviors and step-through latency in mice. We discovered that administration of the extract reduced lipid peroxidation and A aggregation in a biochemical study of mice brain tissues. Treatment with the extract also increased acetylcholine and catalase activity in the brain. Furthermore, the radish extract attenuated HO-induced oxidative stress in cells. Through sequential fractionation of the radish extract, the active compound was identified as oleamide. These results suggest that the radish extract could have a protective role against oxidative stress-induced neuronal toxicity, possibly owing to the antioxidative activity of oleamide.
各种天然化合物已被用于减轻与阿尔茨海默病相关的氧化应激，其中许多化合物可防止细胞受到氧化应激诱导的神经元毒性。在这项研究中，研究了萝卜提取物对小鼠和 PC12 细胞的保护作用。完成了行为测试，以检查提取物对淀粉样β（A）肽诱导的小鼠学习和记忆障碍的保护作用。提取物增加了小鼠的自发交替行为和穿箱潜伏期。我们发现，在对小鼠脑组织的生化研究中，提取物的给药可降低脂质过氧化和 A 聚集。该提取物还增加了大脑中的乙酰胆碱和过氧化氢酶活性。此外，萝卜提取物减轻了 HO 诱导的细胞氧化应激。通过萝卜提取物的顺序分级，鉴定出活性化合物为油酸酰胺。这些结果表明，萝卜提取物可能对氧化应激诱导的神经元毒性具有保护作用，这可能归因于油酸酰胺的抗氧化活性。
