mic基因结构对OmpA系统中阻遏物活性的影响。

Effect of mic gene structure on repressor activity in the OmpA system.

作者信息

Green P J, Mitchell C, Inouye M

出版信息

Biochimie. 1985 Jul-Aug;67(7-8):763-7. doi: 10.1016/s0300-9084(85)80165-6.

Abstract

It has been previously established that micRNA (mRNA-interfering complementary RNA) complementary to an individual mRNA specifically represses the expression of the target mRNA. We have constructed several plasmids which produce micRNAs which are complementary to different regions of the ompA mRNA. The repressor activity of these different micRNAs has been compared to determine the role of mic gene structure in effective micRNA function. The results indicate that micRNAs complementary to regions of the ompA mRNA likely to encounter ribosomes have the highest repressor activities. A clear effect of mic gene dosage was also observed. This was demonstrated using both identical and different mic(ompA) genes.

摘要

先前已经证实，与单个mRNA互补的micRNA（mRNA干扰互补RNA）特异性抑制靶mRNA的表达。我们构建了几种质粒，它们产生与ompA mRNA不同区域互补的micRNA。比较了这些不同micRNA的阻遏活性，以确定mic基因结构在有效micRNA功能中的作用。结果表明，与ompA mRNA中可能遇到核糖体的区域互补的micRNA具有最高的阻遏活性。还观察到mic基因剂量的明显影响。这通过使用相同和不同的mic(ompA)基因得到了证明。