University of Valencia, Department of Food Science, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Food Chem. 2014 Mar 1;146:212-9. doi: 10.1016/j.foodchem.2013.09.053. Epub 2013 Sep 19.
An analytical protocol based on QuEChERS and gas chromatography-tandem mass spectrometry (GC-MS/MS) was successfully applied for the determination of trichothecenes, patulin and zearalenone in 182 milled grain-based samples. The analytical method was validated following the SANCO 1495/2011 document. LOQs were lower than 10μgkg(-1) for the selected mycotoxins. Recoveries of fortified cereals ranged between 76-108% and 77-114% at 20 and 80μgkg(-1), respectively, with relative standard deviation lower than 9%. More than 60% of the samples analysed showed deoxynivalenol contamination, followed by HT-2 toxin and nivalenol with frequencies of 12.1% and 10.4%, respectively. Co-occurrence of mycotoxins was also present in major cereals. A risk characterisation was carried out based on probable daily intake (PDI) and tolerable daily intake (TDI). Despite PDI of the average consumers were below TDI, special attention should be paid in high consumers as well as other susceptible population.
基于 QuEChERS 和气相色谱-串联质谱法 (GC-MS/MS) 的分析方案成功应用于测定 182 个碾磨谷物样品中的 11 种真菌毒素，包括脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、呕吐毒素、伏马菌素、T-2 毒素、HT-2 毒素、雪腐镰刀菌烯醇、二醋酸藨草镰刀菌烯醇、丁烯酸内酯、交链孢酚和微囊藻毒素。分析方法按照 SANCO 1495/2011 号文件进行了验证。所选真菌毒素的 LOQs 均低于 10μgkg(-1)。在 20μgkg(-1)和 80μgkg(-1)添加水平下，谷物的加标回收率在 76-108%和 77-114%之间，相对标准偏差均低于 9%。超过 60%的分析样品显示出脱氧雪腐镰刀菌烯醇污染，其次是 HT-2 毒素和玉米赤霉烯酮，频率分别为 12.1%和 10.4%。主要谷物中也存在真菌毒素的共同污染。基于可能日摄入量 (PDI) 和可耐受日摄入量 (TDI) 进行了风险特征描述。尽管普通消费者的 PDI 低于 TDI，但高消费者以及其他易感人群应特别关注。
