Wang Jingneng, Wang Lihua, Zhang Hui, Mei Xinglin, Qiu Liangzhu, Liu Jing, Zhou Yongsong
Shanghai Xiongtu Biotechnology Co., Ltd., Shanghai, China.
Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, China.
Front Vet Sci. 2023 Mar 16;10:1142820. doi: 10.3389/fvets.2023.1142820. eCollection 2023.
Deoxynivalenol (DON) contamination of food crops and feeds is almost impossible to avoid completely; however, through best management practices, this risk can be effectively managed and maximumly mitigated. Accurate and rapid detection of DON contamination as early in the entire value chain as possible is critical. To achieve this goal, we developed a DON test strip based on time-resolved fluorescence immunoassay (TRFIA) and a specific DON monoclonal antibody for the rapid quantification of DON in food crops and feeds. The strip displayed a good linearity ( = 0.9926), with a limit of quantification of 28.16 μg/kg, a wide linear range of 50 ~ 10,000 μg/kg. The intra-batch coefficient of variation (CV) and the inter-batch CV was <5.00 and 6.60%, respectively. This TRFIA-DON test strip was applied to detect DON in real samples, and the accuracy and reliability were confirmed by liquid chromatography-mass spectrometry (LC-MS/MS). Results showed that the relative standard deviation between the DON strips and LC-MS/MS was <9%. The recovery rates in corn samples ranged from 92 to 104%. The established TRFIA-DON test strip had the characteristics of high sensitivity, high accuracy, and a wide linear range which was suitable for rapid and quantitative determination of DON in food crops and feeds at both on-site and laboratory.
粮食作物和饲料中的脱氧雪腐镰刀菌烯醇（DON）污染几乎不可能完全避免；然而，通过最佳管理实践，这种风险可以得到有效管理并最大程度地减轻。在整个价值链中尽早准确快速地检测DON污染至关重要。为实现这一目标，我们开发了一种基于时间分辨荧光免疫分析（TRFIA）的DON检测试纸条以及一种特异性DON单克隆抗体，用于快速定量粮食作物和饲料中的DON。该试纸条线性良好（ = 0.9926），定量限为28.16 μg/kg，线性范围宽，为50~10,000 μg/kg。批内变异系数（CV）和批间CV分别<5.00%和6.60%。将此TRFIA-DON检测试纸条应用于实际样品中DON的检测，并通过液相色谱-质谱联用（LC-MS/MS）确认了其准确性和可靠性。结果表明，DON试纸条与LC-MS/MS之间的相对标准偏差<9%。玉米样品中的回收率为92%至104%。所建立的TRFIA-DON检测试纸条具有灵敏度高、准确性高、线性范围宽的特点，适用于在现场和实验室快速定量测定粮食作物和饲料中的DON。
