Ningxia Key Laboratory for Food Microbial Applications Technology and Safety Control, Ningxia University, Yinchuan 750021, China.
College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Integrated Research Base of Southern Fruit and Vegetable Preservation Technology, Zhejiang International Scientific and Technological Cooperation Base of Health Food Manufacturing and Quality Control, Zhejiang University, Hangzhou 310058, China.
Molecules. 2022 Aug 15;27(16):5185. doi: 10.3390/molecules27165185.
Fruits of six varieties of young citrus cultivated in China were collected for phytochemical composition analysis and antioxidant activity determination. The phenolic acids, synephrine, flavone, and flavanone were analyzed using HPLC, and the total phenolic content and antioxidant capacity were determined by Folin-Ciocalteu, Ferric ion reducing antioxidant power (FRAP), 2,2- 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) analysis. The results indicated that Ougan variety had the highest total phenolic content (125.18 GAE mg/g DW), followed by the Huyou variety (107.33 mg/g DW), while Wanshuwenzhoumigan variety had the lowest (35.91 mg/g DW). Ferulic acid was the most dominant soluble phenolic acid in the selected young citrus, followed by p-coumaric acid and p-hydroxybenzoic acid, whereas nobiletin and tangeretin were the most abundant flavones in the Ponkan, Ougan, and Wanshuwenzhoumigan varieties. Antioxidant capacity that measured by ABTS, FRAP, and DPPH showed similar trends and was positively correlated with the total phenolic and total flavonoid contents (p < 0.05). Considering the high content of phenolics in the young fruits of Ougan and Huyou variety, those two varieties might be potential resources for extracting phytochemicals for health promotion.
收集了中国栽培的 6 个甜橙品种的幼果，用于分析其植物化学成分和抗氧化活性。采用 HPLC 分析了酚酸、辛弗林、黄酮和黄烷酮，通过福林-考卡尔特、铁离子还原抗氧化能力（FRAP）、2,2-1,1-二苯基-2-苦基肼（DPPH）和 2,2′-联氨-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）分析测定总酚含量和抗氧化能力。结果表明，瓯柑品种的总酚含量最高（125.18 GAE mg/g DW），随后是回游品种（107.33 mg/g DW），而万书文州蜜柑品种的总酚含量最低（35.91 mg/g DW）。阿魏酸是所选幼果中最主要的可溶酚酸，其次是对香豆酸和对羟基苯甲酸，而诺必林和橘红是蓬坎、瓯柑和万书文州蜜柑品种中含量最丰富的黄酮类化合物。ABTS、FRAP 和 DPPH 测定的抗氧化能力呈现出相似的趋势，与总酚和总类黄酮含量呈正相关（p < 0.05）。考虑到瓯柑和回游品种幼果中酚类物质含量较高，这两个品种可能是提取促进健康的植物化学物质的潜在资源。
