Département de Biologic Générale et Appliquée, Université Claude Bernard Lyon I, 43, Boulevard du 11 Novembre 1918, F-69622, Villeurbanne Cedex, France.
Curr Genet. 1984 Jan;8(1):9-13. doi: 10.1007/BF00405424.
The chloroplast DNA of a wild type photosynthetic variant of Euglena gracifs (ATCC n° 10616) with five ribosomal cistrons has been analyzed by restriction mapping. The results complete the electron microscope study of Koller and Delius (MGG 188, 305, 1982); they support a model of formation of the variant DNA by rearrangement of the wild type ribosomal cistrons through unequal crossing-over. The recombination sites have been determined. The recombination model proposed also explains the formation of the "Z-S" variant with a single ribosomal cistron (Wurtz and Buetow 1981).
已通过限制图谱分析对具有五个核糖体基因的野生型光合作用 Euglena gracifs(ATCC n° 10616)的叶绿体 DNA 进行了分析。该结果补充了 Koller 和 Delius 的电子显微镜研究(MGG 188,305,1982);它们支持通过不等交换对野生型核糖体基因进行重排形成变体 DNA 的模型。已确定了重组位点。所提出的重组模型还解释了具有单个核糖体基因的“Z-S”变体的形成(Wurtz 和 Buetow 1981)。