Department of Biochemistry, Antoni van Leeuwenhoek-Huis, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
J Membr Biol. 1972 Dec;9(1):105-26. doi: 10.1007/BF01868047.
Plasma membranes were isolated from rat and mouse livers, one rat hepatoma (and its subline) and two mouse hepatomas, and their lipid class compositions were determined. Lipids accounted for 30 to 35% of the dry weight of the membranes of livers and mouse hepatomas, and for 45% in the case of rat hepatoma-subline. Of the total lipids of rat-liver plasma membranes, 60% consisted of phospholipids, the corresponding values for mouse-liver and rat-hepatoma plasma membranes amounting to 55% and for both mouse-hepatoma plasma membranes to about 50%. The free cholesterol and cholesteryl ester contents of all hepatoma plasma membranes were significantly increased as compared with normal. Evidence is presented that the increase of free cholesterol was not a preparative artefact. The major phospholipid classes in all plasma membranes were phosphatidyl choline, sphingomyelin, phosphatidyl ethanolamine and phosphatidyl serine. The relative proportions in each plasma membrane species could differ appreciably, the mouse- and rat-liver membranes showing the closest resemblance. Possible reasons for (a) the higher level of phosphatidyl serine as compared with published values, and (b) the wide divergencies which may be found among the phospholipid profiles of rat-liver plasma membranes reported by other authors, are presented. Cardiolipin was absent from liver plasma membranes, but some could be found in the hepatoma membranes due to mitochondrial contamination. No consistent phospholipid profile characterized hepatoma as distinct from liver plasma membranes, nor did the hepatoma data-including plasmalogens-resemble the few available data on other hepatomas.
从大鼠和小鼠肝脏、一个大鼠肝癌（及其亚系）和两个小鼠肝癌中分离出质膜，并测定其脂质种类组成。脂质占肝脏和小鼠肝癌质膜干重的 30%至 35%，大鼠肝癌亚系质膜的脂质占 45%。大鼠肝质膜总脂质的 60%由磷脂组成，小鼠肝质膜和大鼠肝癌质膜的相应值为 55%，两个小鼠肝癌质膜的相应值约为 50%。所有肝癌质膜的游离胆固醇和胆固醇酯含量均明显高于正常水平。有证据表明，游离胆固醇的增加不是制备性人为因素所致。所有质膜的主要磷脂类为磷脂酰胆碱、神经鞘磷脂、磷脂酰乙醇胺和磷脂酰丝氨酸。每种质膜种类的相对比例可能有很大差异，小鼠和大鼠肝质膜最为接近。（a）与已发表的值相比，磷脂酰丝氨酸水平较高的原因，以及（b）其他作者报道的大鼠肝质膜磷脂谱可能存在的广泛差异的原因，被提出来讨论。心磷脂不存在于肝质膜中，但由于线粒体污染，一些质膜可以在肝癌质膜中找到。没有一致的磷脂谱可以将肝癌与肝质膜区分开来，肝癌数据（包括质体）也与其他肝癌的少数可用数据不相似。
