Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 151-742, Korea.
Molecules. 2013 Oct 30;18(11):13410-24. doi: 10.3390/molecules181113410.
Due to the widespread and increasing appearance of antibiotic resistance, a new strategy is needed for developing novel antibiotics. Especially, there are no specific antibiotics for Helicobacter pylori (H. pylori). H. pylori are bacteria that live in the stomach and are related to many serious gastric problems such as peptic ulcers, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Because of its importance as a human pathogen, it's worth studying the structure and function of the proteins from H. pylori. After the sequencing of the H. pylori strain 26695 in 1997, more than 1,600 genes were identified from H. pylori. Until now, the structures of 334 proteins from H. pylori have been determined. Among them, 309 structures were determined by X-ray crystallography and 25 structures by Nuclear Magnetic Resonance (NMR), respectively. Overall, the structures of large proteins were determined by X-ray crystallography and those of small proteins by NMR. In our lab, we have studied the structural and functional characteristics of small proteins from H. pylori. In this review, 25 NMR structures of H. pylori proteins will be introduced and their structure-function relationships will be discussed.
由于抗生素耐药性的广泛和不断增加，需要开发新的抗生素策略。特别是，目前没有针对幽门螺杆菌 (H. pylori) 的特定抗生素。H. pylori 是一种生活在胃中的细菌，与许多严重的胃部问题有关，如消化性溃疡、慢性胃炎、黏膜相关淋巴组织淋巴瘤和胃癌。由于其作为人类病原体的重要性，研究 H. pylori 蛋白质的结构和功能是值得的。1997 年 H. pylori 菌株 26695 测序后，从 H. pylori 中鉴定出超过 1600 个基因。到目前为止，已经确定了 334 种 H. pylori 蛋白质的结构。其中，309 种结构通过 X 射线晶体学确定，25 种结构通过核磁共振 (NMR) 确定。总体而言，大蛋白质的结构通过 X 射线晶体学确定，小蛋白质的结构通过 NMR 确定。在我们的实验室中，我们研究了 H. pylori 小蛋白质的结构和功能特征。在这篇综述中，将介绍 25 种 H. pylori 蛋白质的 NMR 结构，并讨论它们的结构-功能关系。
