School of Biological Sciences, The University of Birmingham, B15 2TT, Birmingham, UK.
Theor Appl Genet. 1994 Nov;89(6):698-702. doi: 10.1007/BF00223708.
A method to locate quantitative trait loci (QTL) on a chromosome and to estimate their additive and dominance effects is described. It applies to generations derived from an F1 by selfing or backcrossing and to doubled haploid lines, given that marker genotype information (RFLP, RAPD, etc.) and quantitative trait data are available. The method involves regressing the additive difference between marker genotype means at a locus against a function of the recombination frequency between that locus and a putative QTL. A QTL is located, as by other regression methods, at that point where the residual mean square is minimised. The estimates of location and gene effects are consistent and as reliable as conventional flanking-marker methods. Further applications include the ability to test for the presence of two, or more, linked QTL and to compare different crosses for the presence of common QTL. Furthermore, the technique is straightforward and may be programmed using standard pc-based statistical software.
描述了一种在染色体上定位数量性状基因座（QTL）并估计其加性和显性效应的方法。该方法适用于通过自交或回交从 F1 衍生的世代以及加倍单倍体系，前提是存在标记基因型信息（RFLP、RAPD 等）和数量性状数据。该方法涉及将标记基因型均值的加性差异回归到该位点与假定 QTL 之间重组频率的函数。QTL 的定位与其他回归方法一样，位于残差均方最小化的点。位置和基因效应的估计是一致的，与传统的侧翼标记方法一样可靠。进一步的应用包括能够检测两个或更多连锁 QTL 的存在，并比较不同杂交种中常见 QTL 的存在。此外，该技术简单直接，可以使用标准基于 PC 的统计软件进行编程。
