葡萄（Vitis vinifera L.）的花期调控：离体卷须形成花序。
Control of Flowering in the Grapevine (Vitis vinifera L.): Formation of Inflorescences in Vitro by Isolated Tendrils.
机构信息
Department of Agronomy and Horticultural Science, University of Sydney, New South Wales, 2006, Australia.
出版信息
Plant Physiol. 1978 Jan;61(1):127-30. doi: 10.1104/pp.61.1.127.
Tendrils produced from shoot tips of grapevine (Vitis vinifera L.) cultured in vitro on Nitsch's medium developed into inflorescences when 5 to 10 mum benzyladenine (BA) or 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (PBA) were applied directly to the tendril tips. Inflorescences did not form on tendrils if the cytokinins were supplied in the agar. Tendrils cultured in agitated liquid medium containing BA, PBA, or zeatin riboside showed profuse branching and tendrils were transformed into inflorescences. Calyx and corolla (calyptra) stamens and pistils developed normally in the presence of both zeatin riboside and PBA, but micro- and macrosporogenesis were absent.Inflorescences were formed by tendrils from five cultivars (Muscat of Alexandria, Shiraz, Carbernet Sauvignon, Wortley Hall, and Sultana syn. Thomson Seedless) and also on tendrils from 12- to 15-week-old-seedlings.
在 Nitsch 培养基上培养的葡萄（Vitis vinifera L.）茎尖产生的卷须，当直接在卷须尖端施加 5 到 10 mum 苯并腺嘌呤（BA）或 6-（苄基氨基）-9-（2-四氢吡喃基）-9H-嘌呤（PBA）时，会发育成花序。如果在琼脂中提供细胞分裂素，花序就不会在卷须上形成。在含有 BA、PBA 或玉米素核糖苷的振荡液体培养基中培养的卷须表现出大量分枝，卷须转化为花序。在玉米素核糖苷和 PBA 的存在下，花萼和花瓣（帽状体）雄蕊和雌蕊正常发育，但小孢子和大孢子发生缺失。花序由五个品种（亚历山大麝香葡萄、设拉子、赤霞珠、沃特里厅和苏丹娜 syn. 汤姆森无核）的卷须以及 12 至 15 周龄幼苗的卷须形成。
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