Department of Horticultural Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
Plant Cell Rep. 1996 Mar;15(7):512-5. doi: 10.1007/BF00232984.
Thidiazuron (TDZ) effectively induced somatic embryogenesis in cultured hypocotyl explants of geranium (Pelargonium x hortorum Bailey) during only a 3-day period of induction. The presence of acetylsalicylic acid (ASA) during this period caused a two-fold increase in the number of somatic embryos and enhanced synchronization of embryo development compared to the TDZ treatment alone. Salicylic acid was ineffective in modulating similar embryogenic responses as ASA. The ASA-induced enhancement and synchronization of somatic embryogenesis could possibly be used as an experimental system to study the interplay of growth regulators in somatic embryogenesis.
噻二唑苯基脲(TDZ)在仅 3 天的诱导期内,有效地诱导天竺葵(Pelargonium x hortorum Bailey)培养下胚轴外植体的体细胞胚胎发生。在此期间,乙酰水杨酸(ASA)的存在使体细胞胚的数量增加了一倍,并与单独使用 TDZ 处理相比,增强了胚胎发育的同步性。水杨酸在调节类似的胚胎发生反应方面无效。ASA 诱导的体细胞胚胎发生的增强和同步性可能可被用作研究生长调节剂在体细胞胚胎发生中的相互作用的实验系统。