Felińska W, Szkilnik R, Stanosek B, Kamiński M, Ludyga K, Brus R
Med Pr. 1985;36(4):236-43.
The experiment was carried out on male guinea pigs exposed to NO2 at a constant concentration of 0.1 ppm 24 h daily for 1, 2.5 and 6 months, or NO2 concentration was increased to 0.5 ppm twice daily, between 7:00 and 8:00 AM and 4:00 and 5:00 PM. After the exposure to NO2 was over the animals were killed by decapitation. The cranium was opened and the brain removed, and afterwards at 0 degrees C the left hemisphere was dissected into discrete areas which were then frozen on dry ice and weighed. The tissues were kept for 2-3 days at -20 degrees C, and afterwards the content of noradrenaline, 5-hydroxytryptamine and 5-hydroxyindole-acetic acid was estimated spectrofluorometrically in examined parts of the brain (thalamus--hypothalamus pons + medulla oblongata hippocampus, cortex frontalis). It was shown that the applied NO2 concentrations brought about slight and nonspecific alterations in the content of the investigated amines in guinea pigs' brain.
实验以雄性豚鼠为对象，使其每天24小时持续暴露于浓度为0.1 ppm的二氧化氮环境中，分别暴露1个月、2.5个月和6个月；或者每天两次将二氧化氮浓度提高到0.5 ppm，暴露时间为上午7:00至8:00以及下午4:00至5:00。二氧化氮暴露结束后，将动物断头处死。打开颅骨取出大脑，然后在0摄氏度下将左半球切成不同区域，随后在干冰上冷冻并称重。组织在-20摄氏度下保存2至3天，之后采用荧光分光光度法测定大脑各检查部位（丘脑 - 下丘脑、脑桥 + 延髓、海马体、额叶皮质）中去甲肾上腺素、5-羟色胺和5-羟吲哚乙酸的含量。结果表明，所采用的二氧化氮浓度使豚鼠大脑中所研究胺类的含量发生了轻微的非特异性变化。
