Ma J-H, Yao J-L, Cohen D, Morris B
The Horticulture and Food Research Institute of New Zealand, Mount Albert Research Centre, Private Bag 92169, Auckland, New Zealand Fax no.: +64-9-815-4201 E-mail:
Plant Cell Rep. 1998 Jan;17(3):211-214. doi: 10.1007/s002990050380.
Effects of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and three ethylene inhibitors, AgNO, aminoethoxyvinyglycine (AVG) and CoCl, on root formation were tested in vitro using shoot cultures of the apple (Malus×domestica Borkh.) cultivar Royal Gala. ACC inhibited root formation by delaying root emergence and increasing callus formation at the bases of shoots. In contrast, ethylene inhibitors promoted root formation. Both AgNO and AVG at the appropriate concentrations increased the percentage of shoots producing roots and reduced callus formation at the base of these shoots. AgNO stimulated root emergence and enhanced root growth, while AVG increased the number of roots per shoot. CoCl slightly increased root number and rooting efficiency. These promotive effects may result from a reduction in ethylene concentration or inhibition of ethylene action. The results found in this study may be used to improve the rooting efficiency of other apple cultivars and rootstocks, and possibly of other plant species.
利用苹果（Malus×domestica Borkh.）品种皇家嘎拉的茎段培养物，在体外测试了乙烯前体1-氨基环丙烷-1-羧酸（ACC）和三种乙烯抑制剂（硝酸银、氨基乙氧基乙烯基甘氨酸（AVG）和氯化钴）对生根的影响。ACC通过延迟根的出现和增加茎基部愈伤组织的形成来抑制生根。相反，乙烯抑制剂促进生根。适当浓度的硝酸银和AVG都增加了生根茎段的百分比，并减少了这些茎段基部的愈伤组织形成。硝酸银刺激根的出现并促进根的生长，而AVG增加了每个茎段的根数。氯化钴略微增加了根数和生根效率。这些促进作用可能是由于乙烯浓度降低或乙烯作用受到抑制。本研究的结果可用于提高其他苹果品种和砧木的生根效率，也可能用于提高其他植物物种的生根效率。
