New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand.
Theor Appl Genet. 1994 Sep;88(8):1050-5. doi: 10.1007/BF00220815.
Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).
利用连锁分析确定了豌豆中隐性抗白粉病基因 er-1 的遗传图谱定位。在分析两个杂交后代（F2 群体和一组重组自交系）后，发现 er-1 与连锁群 6 标记之间存在遗传连锁。er-1 周围的遗传标记包括 RFLP、RAPD 和同工酶标记以及形态标记 Gty。通过混池分离分析鉴定到与 er-1 紧密连锁的 RAPD 标记。在 DNA 序列特征分析后，设计了特异性 PCR 引物将此 RAPD 标记转化为序列特征扩增区（SCAR）。
