Sub-Department of Environmental Technology, Wageningen University, Bornse Weilanden 9, 6700 AA Wageningen, The Netherlands.
Archaea. 2013;2013:481784. doi: 10.1155/2013/481784. Epub 2013 Sep 25.
A methane-producing biocathode that converts CO(2) into methane was studied electrochemically and microbiologically. The biocathode produced methane at a maximum rate of 5.1 L CH(4)/m(2) projected cathode per day (1.6 A/m(2)) at -0.7 V versus NHE cathode potential and 3.0 L CH(4)/m(2) projected cathode per day (0.9 A/m(2)) at -0.6 V versus NHE cathode potential. The microbial community at the biocathode was dominated by three phylotypes of Archaea and six phylotypes of bacteria. The Archaeal phylotypes were most closely related to Methanobacterium palustre and Methanobacterium aarhusense. Besides methanogenic Archaea, bacteria seemed to be associated with methane production, producing hydrogen as an intermediate. Biomass density varied greatly with part of the carbon electrode covered with a dense biofilm, while only clusters of cells were found on other parts. Based on our results, we discuss how inoculum enrichment and changing operational conditions may help to increase biomass density and to select for microorganisms that produce methane.
一种能够将二氧化碳转化为甲烷的产甲烷生物阴极，通过电化学和微生物学方法进行了研究。在-0.7 V 相对于 NHE 阴极电位时，生物阴极以每天 5.1 L CH4/ m2 预测阴极的最大速率（1.6 A/m2）产生甲烷，在-0.6 V 相对于 NHE 阴极电位时以每天 3.0 L CH4/ m2 预测阴极的最大速率（0.9 A/m2）产生甲烷。生物阴极上的微生物群落主要由三种古菌和六种细菌的菌型组成。古菌菌型与沼泽甲烷杆菌（Methanobacterium palustre）和 Aarhus 甲烷杆菌（Methanobacterium aarhusense）最为密切相关。除产甲烷古菌外，似乎还有细菌与甲烷的产生有关，它们将氢气作为中间产物产生。生物量密度变化很大，部分碳电极被密集的生物膜覆盖，而其他部分只发现细胞簇。基于我们的结果，我们讨论了接种物富集和改变操作条件如何有助于增加生物量密度，并选择产生甲烷的微生物。
