基于死亡和存活人群之间的比较进行长寿相关基因的筛选。
Screening of longevity-associated genes based on a comparison between dead and surviving populations.
机构信息
Dalian Blood Center, China; College of Medical Laboratory, Dalian Medical University, China.
Dalian Blood Center, China.
出版信息
Gene. 2014 Jan 25;534(2):379-82. doi: 10.1016/j.gene.2013.08.070. Epub 2013 Nov 2.
AIM
To investigate longevity-associated genes based on a comparison between dead and surviving populations.
METHODS
A total of 71 cases of dead individuals were treated as the death group, and healthy volunteers who were matched with the dead individuals based on sex and age were recruited as the survival group. Alleles of 13 CODIS STR loci were determined using the AmpFLSTR Profiler Plus PCR Amplification Kit. The cross-validation was performed based on differences between the two groups in both frequency values and ages.
RESULTS
The frequency value of the D18S51-17 alleles was significantly higher in the dead group than in the survival group (p<0.05), and the frequency value of the D2S1338-18 allele was statistically lower in the dead group than in the survival group (p<0.05). The mean age of the subjects with the D2S1338-18 allele was also significantly higher than that of the subjects without D2S1338-18, and no significant difference was observed with respect to the other three alleles.
CONCLUSIONS
The results suggest that D2S1338-18 is associated with longevity.
目的
基于死亡人群和存活人群的比较，来研究与长寿相关的基因。
方法
共选取 71 例死亡个体作为死亡组，选取与死亡个体在性别和年龄上相匹配的健康志愿者作为存活组。使用 AmpFLSTR Profiler Plus PCR 扩增试剂盒确定 13 个 CODIS STR 基因座的等位基因。基于两组间在频率值和年龄上的差异进行交叉验证。
结果
死亡组中 D18S51-17 等位基因的频率值显著高于存活组（p<0.05），而 D2S1338-18 等位基因的频率值在死亡组中显著低于存活组（p<0.05）。携带 D2S1338-18 等位基因的受试者的平均年龄也显著高于不携带 D2S1338-18 等位基因的受试者，而其他三个等位基因则没有观察到显著差异。
结论
研究结果提示 D2S1338-18 与长寿有关。
Zotero 插件