牛无形体的系统发育位置及无形体属系统发育推断
The phylogenetic position of Anaplasma bovis and inferences on the phylogeny of the genus Anaplasma.
作者信息
Ybañez Adrian Patalinghug, Sashika Mariko, Inokuma Hisashi
机构信息
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
出版信息
J Vet Med Sci. 2014 Mar 1;76(2):307-12. doi: 10.1292/jvms.13-0411. Epub 2013 Nov 1.
The present study aimed to determine the complete citrate synthase (gltA) and heat-shock protein (groEL) gene sequences of Anaplasma bovis and to infer phylogenetic relationships within the genus Anaplasma. Multiple alignments from single and concatenated sequences of the 16S rRNA, gltA and groEL genes of the genus Anaplasma were subjected to phylogenetic analyses. Percent identities of A. bovis nucleotide sequences were found highest with A. phagocytophilum in gltA (65.4%) and groEL (79.8%). Single gene phylogenetic tree results assumed similar phylogenetic positions within the genus Anaplasma, except for A. bovis. However, consensus and concatenated sequence phylogenetic trees showed similar results, revealing 2 subgroups within the genus.
本研究旨在确定牛无形体的柠檬酸合酶（gltA）和热休克蛋白（groEL）基因的完整序列，并推断无形体属内的系统发育关系。对无形体属16S rRNA、gltA和groEL基因的单序列和串联序列进行多重比对，然后进行系统发育分析。发现牛无形体核苷酸序列与嗜吞噬细胞无形体在gltA（65.4%）和groEL（79.8%）上的百分比一致性最高。单基因系统发育树结果显示，除牛无形体外，在无形体属内假定处于相似的系统发育位置。然而，一致序列和串联序列系统发育树显示出相似的结果，揭示了该属内的2个亚组。
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