Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia; Department of Basic Sciences, School of Medicine, Copperbelt University, Kitwe, Zambia.
Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.
Ticks Tick Borne Dis. 2018 Jan;9(1):39-43. doi: 10.1016/j.ttbdis.2017.10.010. Epub 2017 Oct 17.
Although tick-borne pathogens, Anaplasma platys and Anaplasma phagocytophilum are recognized as zoonotic agents associated with appreciable morbidity and mortality in dogs and humans worldwide, there is limited information on these infections in many African countries, including Zambia. The purpose of this study was to detect, identify and phylogenetically characterize Anaplasma species from dogs in Chilanga District in Lusaka Province, Zambia. A total of 301 blood samples were collected from apparently healthy and semi-confined dogs. Initial screening by polymerase chain reaction with specific primers targeting the 16S rRNA gene of Anaplasma species revealed that 9% (27/301) of our samples were positive. Subsequent sequence and phylogenetic analysis of a longer fragment of the 16S rRNA and citrate synthase (gltA) genes of four positive samples showed the presence of A. platys and an Anaplasma species, which was closely related to those detected in dogs in South Africa. This is the first report on molecular identification and characterization of canine-associated zoonotic Anaplasma species in Zambia.
虽然蜱传病原体阿氏巴通体和嗜吞噬细胞无形体被认为是与全球犬和人类的相当发病率和死亡率相关的动物源性传染病病原体，但在包括赞比亚在内的许多非洲国家，有关这些感染的信息有限。本研究的目的是从赞比亚卢萨卡省奇兰加区的犬中检测、鉴定和系统发育分析阿氏巴通体种。从看似健康和半封闭的犬中采集了 301 份血样。使用针对阿氏巴通体 16S rRNA 基因的特异性引物进行聚合酶链反应的初步筛查显示，我们的样本中有 9%（27/301）为阳性。随后对 4 个阳性样本的 16S rRNA 和柠檬酸合酶（gltA）基因的较长片段进行序列和系统发育分析表明，存在阿氏巴通体和一种与在南非犬中检测到的密切相关的阿氏巴通体种。这是赞比亚首次报道犬相关动物源性传染病阿氏巴通体种的分子鉴定和特征。
