Department of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, 080-8555 Obihiro, Hokkaido, Japan.
Vet Microbiol. 2012 May 25;157(1-2):232-6. doi: 10.1016/j.vetmic.2011.12.001. Epub 2011 Dec 11.
An Anaplasma species closely related to Anaplasma phagocytophilum detected in sika deer in Hokkaido, Japan was molecularly analyzed using 16S rRNA, citrate synthase (gltA), and heat-shock operon (groEL) gene sequences. Genome walking was performed to determine its complete gltA and groEL sequences (1233 bp and 1650 bp, respectively). Percent identities to the closest A. phagocytophilum sequences from the US and European strains were 98.6-98.8%, 76.5%, and 80.3-80.8% for 16S rRNA, gltA, and groEL genes, respectively. For deduced amino acid sequences, percent identities to the closest A. phagocytophilum sequences were 66.7% and 97.6% for gltA and groEL genes, respectively. Phylogenetic analyses revealed divergence from any known A. phagocytophilum strain. The lower identities and the divergent phylogenetic position of the Anaplasma sp. detected from sika deer in Japan with established A. phagocytophilum strains provide evidence of its potential novelty.
在日本北海道的梅花鹿中检测到一种与嗜吞噬细胞无形体密切相关的无形体种，使用 16S rRNA、柠檬酸合酶（gltA）和热休克操纵子（groEL）基因序列对其进行了分子分析。进行了基因组步移以确定其完整的 gltA 和 groEL 序列（分别为 1233bp 和 1650bp）。与来自美国和欧洲株的最接近的嗜吞噬细胞无形体序列的 16S rRNA、gltA 和 groEL 基因的百分比同一性分别为 98.6-98.8%、76.5%和 80.3-80.8%。对于推导的氨基酸序列，gltA 和 groEL 基因与最接近的嗜吞噬细胞无形体序列的百分比同一性分别为 66.7%和 97.6%。系统发育分析表明，该无形体种与任何已知的嗜吞噬细胞无形体株均有分歧。从日本梅花鹿中检测到的与已建立的嗜吞噬细胞无形体株不同的无形体种的较低同一性和分化的系统发育位置提供了其潜在新颖性的证据。
