School of Biological Sciences, Flinders University, GPO 2100, 5001, Adelaide, Australia.
Microb Ecol. 1993 Mar;25(2):113-9. doi: 10.1007/BF00177189.
A wide variety of naturally occurring bacteria cannot be isolated by classical microbiological methods, hindering study of microbial communities. To partially remove this limitation, a method using optical trapping was developed. It allows for one-step isolation of bacteria from a complex community to a pure culture. The method transfers a single bacterium to sterile culture medium using a non-destructive laser beam. The ease with which a previously unculturable cell was cultured by trapping suggests that competition may limit growth in some natural communities of bacteria.
目前有多种自然存在的细菌无法通过传统的微生物学方法进行分离，这对微生物群落的研究造成了阻碍。为了部分克服这一限制，人们开发了一种利用光学捕获的方法。该方法可以一步将细菌从复杂的群落中分离到纯培养物中。该方法使用非破坏性激光束将单个细菌转移到无菌培养基中。通过捕获可以轻松培养以前无法培养的细胞，这表明竞争可能会限制某些自然细菌群落中的生长。
