Department of Plant Pathology, University of Wisconsin, 1630 Linden Drive, 53706, Madison, WI, USA.
Theor Appl Genet. 1993 Dec;87(5):545-57. doi: 10.1007/BF00221877.
A genetic map of Magnaporthe grisea (anamorph=Pyricularia oryzae and P. grisea), the causal agent of rice blast disease, was generated from segregation data utilizing 97 RFLP markers, two isoenzyme loci and the mating type locus among progeny of a cross between parental strains Guy 11 and 2539. Of the seven chromosomes of M. Grisea, three were resolved by contour-clamped homogeneous electric field (CHEF) electrophoresis, while the remaining four migrated as two doublet bands. By utilizing differences between CHEF mobilities of unresolved chromosomes from the parental strains, Southern analysis with selected markers allowed the chromosomal assignment of all linkage groups. A small translocation involving 1 marker was found in the parental strains used to produce the segregating population from which the map was constructed. Nine classes of repetitive DNA elements were found in the genome of a fungal isolate pathogenic to rice. These occurred only a few times or not at all in the genomes of isolates showing reduced virulence on rice. One repetitive DNA was shown to have structural similarity to the Alu sequences found in primates, a sequence similarity to the copia-like elements of Drosophila, and peptide similarity to transposable elements found in Drosophila, other fungi, and higher plants.
稻瘟病菌（无性型为玉蜀黍赤霉和稻梨孢）的遗传图谱是利用杂种 Guy11 和 2539 后代的 97 个 RFLP 标记、2 个同工酶位点和交配型位点的分离数据生成的。在稻瘟病菌的 7 条染色体中，3 条通过等电聚焦电泳（CHEF）电泳得到解析，而其余 4 条则以两个双联体带的形式迁移。通过利用亲本菌株未解析染色体的 CHEF 迁移率之间的差异，利用选定的标记进行 Southern 分析可以对所有连锁群进行染色体分配。在所构建的分离群体的亲本菌株中发现了一个涉及 1 个标记的小易位。在对水稻致病的真菌分离物的基因组中发现了 9 类重复 DNA 元件。在对水稻致病力降低的分离物的基因组中，这些元件只出现了几次或根本没有出现。一个重复 DNA 与灵长类动物中的 Alu 序列、果蝇中的 copia-like 元件以及果蝇、其他真菌和高等植物中的转座元件具有结构相似性。
