Fujian Universities Key Laboratory for Plant-Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Sci Data. 2024 Oct 31;11(1):1186. doi: 10.1038/s41597-024-04035-z.
Fungi from the Pyricularia genus cause blast disease in many economically important crops and grasses, such as wheat, rice, and Cenchrus grass JUJUNCAO. Structure variation associated with the gain and loss of effectors contributes largely to the adaptive evolution of this fungus towards diverse host plants. A telomere-to-telomere genome assembly would facilitate the identification of genome-wide structural variations through comparative genomics. Here, we report a telomere-to-telomere, near-complete genome assembly of a Pyricularia penniseti isolate JC-1 infecting JUJUNCAO. The assembly consists of eight core chromosomes and two supernumerary chromosomes, named mini1 and mini2, spanning 42.1 Mb. We annotated 12,156 protein-coding genes and identified 4.54% of the genome as repetitive sequences. The two supernumerary chromosomes contained fewer genes and more repetitive sequences than the core chromosomes. Our genome and results provide valuable resources for the future study in genome evolution, structure variation and host adaptation of the Pyricularia fungus.
层出孔菌属真菌可引起多种重要经济作物和草类的稻瘟病，如小麦、水稻和狼尾草。与效应子获得和丧失相关的结构变异在很大程度上促进了该真菌对不同宿主植物的适应性进化。端粒到端粒的基因组组装将通过比较基因组学促进全基因组结构变异的鉴定。在这里，我们报告了一个感染狼尾草的稻瘟病菌 JC-1 的端粒到端粒的近完整基因组组装。该组装由 8 个核心染色体和两个超数染色体，命名为 mini1 和 mini2，共包含 42.1Mb。我们注释了 12156 个蛋白编码基因，并鉴定出 4.54%的基因组为重复序列。两个超数染色体比核心染色体包含更少的基因和更多的重复序列。我们的基因组和结果为稻瘟病菌的基因组进化、结构变异和宿主适应性的未来研究提供了有价值的资源。
