Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, Scotland, UK.
Theor Appl Genet. 1993 Mar;86(1):71-80. doi: 10.1007/BF00223810.
The taxonomic relationships between 52 accessions of 12 Vicia species and three accessions of Lathyrus were examined using nuclear RFLP- and PCR-generated data. Two hundred and sixty informative restriction fragments or amplification products were analysed by single linkage analysis, average cluster analysis, and the Wagner parsimony method. Dendrograms constructed from each type of analysis showed similar overall topologies and could be divided into three parts corresponding respectively to the Lathyrus outgroup, the species grouped in the section Faba/narbonemis complex, and the species belonging to the sections Hypechusa and Peregrinae. With few exceptions, the majority of accessions belonging to one species grouped together before branching to other species. An analysis of mitochondrial DNA phenotypes was both consistent with and complemented the results from the nuclear data. Overall, the species relationships show a good correlation with the classification of Maxted et al. but suggest that V. faba is more closely aligned to species from the sections Hypechusa and Peregrinae than to those in the narbonensis complex. In addition, the position of two new species, V. kalakhensis and V. eristaloides, as members of the narbonensis complex was supported by the molecular data, which also allowed a preliminary classification for recently collected Vicia accessions.
使用核 RFLP 和 PCR 生成的数据，对 12 种 Vicia 物种的 52 个品系和 3 个 Lathyrus 品系进行了分类关系研究。通过单链接分析、平均聚类分析和 Wagner 简约法分析了 260 个信息丰富的限制片段或扩增产物。每种分析方法构建的系统发育树显示出相似的总体拓扑结构，可分为三部分，分别对应于 Lathyrus 外群、归属于 Fabo/narbonemis 复合体的物种、以及归属于 Hypechusa 和 Peregrinae 部分的物种。除了少数例外，大多数属于一个物种的品系在分支到其他物种之前先聚在一起。线粒体 DNA 表型分析与核数据的结果一致且互为补充。总体而言，物种关系与 Maxted 等人的分类法有很好的相关性，但表明 V. faba 与 Hypechusa 和 Peregrinae 部分的物种比与 narbonensis 复合体的物种更为接近。此外，两个新物种 V. kalakhensis 和 V. eristaloides 作为 narbonensis 复合体的成员的地位得到了分子数据的支持，这些数据还允许对最近收集的 Vicia 品系进行初步分类。
