Departamento de Genética, E.T.S.I.A.M., Aptdo. 3048, 14080, Córdoba, Spain.
Theor Appl Genet. 1996 Feb;92(2):273-7. doi: 10.1007/BF00223385.
Nineteen species of rose (Rosa sp.) were analysed using Random Amplified Polymorphic DNA markers (RAPD). Each 10-base-long arbitrary primer produced a specific DNA banding pattern that grouped plants belonging to the same species and botanical sections as predicted from their genetic background. One hundred and seventy-five amplification products were examined by cluster analysis to assess the genetic relationships among species and their genetic distances. All of the accessions belonging to 1 species grouped together before branching to other species. Dendrograms constructed for intra- and inter-specific studies showed a good correlation with previous classifications by different authors based on morphological and cariological studies. Our results show that the RAPD technique is a sensitive and precise tool for genomic analysis in rose, being useful in assigning unclassified accessions to specific taxonomic groups or else allowing accessions classified by traditional criteria to be re-classified.
采用随机扩增多态性 DNA 标记（RAPD）分析了 19 种玫瑰（Rosa sp.）。每种 10 个碱基长的任意引物产生特定的 DNA 带型，根据遗传背景将属于同一物种和植物科的植物分组。通过聚类分析检查了 175 个扩增产物，以评估物种间的遗传关系及其遗传距离。属于 1 个物种的所有材料在分支到其他物种之前先聚集成组。构建的种内和种间系统发育树与不同作者基于形态学和细胞遗传学研究的先前分类结果具有良好的相关性。我们的结果表明，RAPD 技术是玫瑰基因组分析的一种敏感和精确的工具，可用于将未分类的材料分配到特定的分类群中，或者允许根据传统标准分类的材料重新分类。
