Bosmali Irene, Lagiotis Georgios, Haider Nadia, Osathanunkul Maslin, Biliaderis Costas, Madesis Panagiotis
Centre for Research and Technology Hellas, Institute of Applied Biosciences, 57001 Thessaloniki, Greece.
Laboratory of Food Chemistry and Biochemistry, Department of Food Science and Technology, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Plants (Basel). 2022 Mar 31;11(7):947. doi: 10.3390/plants11070947.
Many legume species of the L. genus (Fabaceae Lindl.) are key components of the Mediterranean diet and have an integral role in sustainable agriculture. Given the importance of the species for Eurasian culture, it is necessary to implement methodologies, such as DNA barcoding, that can enable the effective authentication and identification of species in the genus. In this study, we analysed the chloroplast and , as well as the nuclear ITS2 DNA barcoding regions, to identify 71 specimens of Eurasian descent. Both the and ITS2 regions were highly effective in discriminating the analysed taxa, while the more conserved region could not identify all of the selected species due to high sequence conservation or non-annotated or absent species sequences in GenBank. A dendrographic representation of the generated data showed sufficient clustering for most of the analysed taxa, although some topological discrepancies were observed. ITS2 and reconstructions were also used for resolving the topological discrepancies observed in the tree. Our analysis suggests that a combination of DNA barcoding regions is essential for accurate species discrimination within the genus, while single-locus analyses do not provide the necessary resolution.
豆科蝶形花亚科百脉根属的许多豆科植物物种是地中海饮食的关键组成部分，在可持续农业中发挥着不可或缺的作用。鉴于该物种对欧亚文化的重要性，有必要采用诸如DNA条形码等方法，以实现对该属物种的有效鉴定和识别。在本研究中，我们分析了叶绿体[具体基因未给出]以及核ITS2 DNA条形码区域，以鉴定71个欧亚血统的标本。[具体基因未给出]区域和ITS2区域在区分所分析的分类群方面都非常有效，而由于序列保守性高或GenBank中未注释或缺少[具体物种未给出]物种序列，更为保守的[具体基因未给出]区域无法识别所有选定的物种。生成的[具体基因未给出]数据的系统发育树状图显示，大多数分析的分类群有足够的聚类，尽管观察到一些拓扑差异。ITS2和[具体基因未给出]重建也用于解决在[具体基因未给出]树中观察到的拓扑差异。我们的分析表明，DNA条形码区域的组合对于百脉根属内准确的物种区分至关重要，而单基因座分析无法提供必要的分辨率。
