Institute of Botany, Ludwig-Maximilians-University Munich, Menzinger Strasse 67, 19, Munich, Germany.
Theor Appl Genet. 1993 May;86(4):449-57. doi: 10.1007/BF00838560.
Forty-one accessions of the genusBeta representing wild and cultivated species of all sections were analyzed by DNA "fingerprinting". Four sugar beet minisatellite DNA probes revealed characteristic banding patterns with Southern-hybridizedBeta DNA restricted withHindIII. A total of 111 polymorphic RFLP bands were scored across all accessions. Cluster analysis based on genetic similarity estimates for all 820 combinations of accessions revealed the following results. (1) All accessions could unambiguously be identified by a characteristic RFLP banding pattern. (2) The sugar beet cultivars examined displayed a low level of genetic diversity; they showed high similarity toB. Vulgaris ssp.maritima but low genetic similarity to the other wild species of section I. (3) In most cases, the present taxonomic classification of the genusBeta was confirmed. Species of sections II, III, and IV were clearly distinguishable from those of section I except forB. Macrocarpa, which showed high similarity to wild species of section II. In a second experiment, 108 single-copy RFLP probes from sugar beet were Southern hybridized withB. procumbens DNA. A surprisingly low degree of homology (34%) was found. The results are discussed with regard to the taxonomic classification of the genusBeta.
对 41 个代表所有节的野生和栽培物种的 Beta 属进行了 DNA“指纹”分析。四种甜菜微卫星 DNA 探针揭示了与 Southern 杂交的 Beta DNA 用 HindIII 限制的特征带型。在所有供体中总共记录了 111 个多态性 RFLP 带。基于所有 820 个供体组合的遗传相似性估计的聚类分析得出以下结果。(1) 所有供体都可以通过特征性的 RFLP 带型图谱来明确识别。(2) 所检查的甜菜品种显示出低水平的遗传多样性；它们与 B. vulgaris ssp.maritima 高度相似，但与第一节的其他野生种的遗传相似性较低。(3) 在大多数情况下，确认了 Beta 属的现行分类。第二节、第三节和第四节的物种与第一节的物种明显不同，除了 B. Macrocarpa，它与第二节的野生种高度相似。在第二个实验中，108 个来自甜菜的单拷贝 RFLP 探针与 B. procumbens DNA 进行了 Southern 杂交。发现同源性非常低（34%）。结果与 Beta 属的分类学分类有关。
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