Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602-7273, Athens, GA, USA.
Plant Cell Rep. 1994 Jan;13(3-4):159-63. doi: 10.1007/BF00239884.
Somatic embryos were produced from peanut (Arachis hypogaea L.) immature zygotic cotyledons. Comparisons were made of the level of α-naphthaleneacetic acid during induction, nitrogen formulation of the medium, and photoperiod. Over 70% embryogenesis was obtained regardless of NAA level used. Percent embryogenesis and number of embryos were markedly lower in explants induced on NAA compared to 2,4-D. Embryo production was not greatly affected by either the use of Murashige & Skoog versus Finer & Nagasawa salts or light versus dark culture conditions. However, embryo morphology was noticeably affected by photoperiod. Embryos produced under a 16 h photoperiod were tough, woody and difficult to separate for subsequent germination and conversion. Those produced under a 0-h photoperiod were succulent and pliable.
体细胞胚胎从花生（Arachis hypogaea L.）未成熟的合子子叶中产生。比较了诱导过程中α-萘乙酸的水平、培养基的氮配方和光周期。无论使用的 NAA 水平如何，胚胎发生率都超过 70%。与 2,4-D 相比，在 NAA 诱导的外植体中，胚胎发生率和胚胎数量明显较低。胚胎的产生受 Murashige 和 Skoog 与 Finer 和 Nagasawa 盐的使用以及光照与黑暗培养条件的影响不大。然而，胚胎形态明显受光周期的影响。在 16 小时光周期下产生的胚胎坚硬、木质化，难以进行后续的发芽和转化。在 0 小时光周期下产生的胚胎多汁且柔韧。
